CHOK1-GenS&CHOK1-ADCC+

Cell Line Development System Commercial License

ProBio has 12 years of experience in mammalian cell line development, and is one of the earliest companies getting the CHO-K1 - CHO commercial license for commercial use and sublicense agreement from ECACC.

ProBio has two cell line development systems: wild-type cell line CHOK1-GenS and ADCC-enhanced cell line CHOK1-ADCC +. CHOK1-GenS cell line development system without chemical . Developed ADCC enhanced host cell line (CHOK1-ADCC+), which can produce afucosylated monoclonal antibodies with enhanced ADCC activity. They are in together with the high-expression proprietary pGenHT 1.0-UP vector, the average titer of mAb is 5.7 g/L, the maximum titer of mAb is up to 15 g/L.

CHOK1 system exclusive advantages

IP Clear

• ECACC license agreement• Proprietary pGenHT 1.0-UP vector

Royalty Free

• One-time payment without royalty

2 CHO-K1 Host Cell License to Choose

• Wide type CHOK1-GenS• ADCC enhanced CHOK1-ADCC+

Combo license at discounted price

• Purchase in combination with wild type CHOK1-GenS cell line, and get a discounted price

Suitable for different type of proteins

• mAb/Protein

• BsAb,etc

Fulfill the regulatory requirements

• Fulfill the regulatory requirements of FDA,EMA and NMPA

Case Study of CHOK1-GenS cell line system

High productivity with CHOK1-GenS system

• Suitable for different type of molecules

 Excellent stability with CHOK1-GenS system

• The titer at the end of stability study during fed-batch is recorded as T2.
• The titer at the beginning of stability study during fed-batch is recorded as T1.
• Titer variation=(T2-T1)/T1*100%
• Titer variation should be within 30%.

High productivity with CHOK1-GenS system

Titer prior to cell culture up to 15 g/L

Excellent Stability in Scale-Up from 3L to 200L

Case Study of CHOK1-ADCC+ cell line system

CD16a 158V/F affinity (BLI)

The affinity to CD16a (158V and 158F) was increased by 3-4 times.

PBMC-based ADCC

• Cytotoxicity is increased by about 10%.
• Effect concentration (EC50) was reduced by 6 times.
• ADCC effect was significantly enhanced.

CHOK1 delivery package

Media Contact
Company Name: ProBio CDMO
Email: Send Email
Phone: 1-877-436-7274 (Toll-Free)
Country: United States
Website: https://www.probiocdmo.com